The effect of paperboard bacterial culture (PBC) supplementation during fermentative hydrogen production under the variation of initial substrate concentration to inoculum (S/X) ratio was studied. The paperboard mill wastewater (PMW) has been used as substrate. The results showed that supplementation of heat-pretreated PBC to PMW substantially improved the hydrogen productivity. Where, the hydrogen yield (HY) increased from 1.72 to 2.63 mmol/gCODinitial by supplementation of PBC to PMW compared to PMW as a sole substrate, with a peak hydrogen production (HP) of 2.4 mmol. Moreover, HP at S/X ratio of 0.3 gCOD/gVS resulted in the highest value of 3.8 mmol, where lower and higher S/X ratios led to deterioration in HP in the range of (65 to 77%). Meanwhile, the highest % soluble/total carbohydrates removal of 72.73% was corresponded to the highest HY (2.1 mmol H2/gCODinitial) and % removal of VSS (63.16%) at 0.3 gCOD/gVS. This indicates that most of produced H2 was generated from the conversion of organic particulates in PMW. The main soluble metabolites analysis showed the mixed acetate-butyrate fermentation type was occurred with small concentrations of propionate and lactate.
Ahmed M. Farghaly, Egypt-Japan University of Science and Technology (E-JUST), Egypt
Ahmed Tawfik, Egypt-Japan University of Science and Technology (E-JUST), Egypt
Stream: Environmental Sustainability & Human Consumption: Waste
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