Lignocellulosic biomass is a renewable, inexpensive, and abundant resource with high potential for biofuel production to implement the sustainable energy energy worldwide. The bottleneck of biofuel production is the recalcitration of ligoncellulosic biomass to fermentable sugars. Searching for a novel cellulase, a biocatalyst, is one of major challenges to promote biofuel production with economic and environmental friendly. Natural microorganism is the great source of cellulase production. Therefore the objective of this research is to identify thermophilic, rapid, efficient cellulose-degrading bacteria from organic fertilizer, rice field, activated sludge, and rain forest in Thailand. More than 250 isolates were screened at 55°C on carboxy-methyl-cellulose(CMC)-containing media to observe the cellulase activity. Using standard filter paper assay, only 9 isolates (T7, T10, T20, M2-2, M3-1, M11-3, M2-1, M11-2, M11-4) showed high total cellulase compared to the positive control (Cellulomonas sp). A portion of the 16srDNA genes of cellulase positive isolates were amplified and sequenced, then BLASTed to determine species. All isolates then were cultured in media containing 4 different carbon sources; CMC, filter paper, rice straw, nutrient broth (NB), to see effect of substrates on cellulase production. It showed that CMC and NB could induce high total cellulase activity in the most of all isolates up to 1,944 and 1,392 uM of glucose equivalents respectively which is greater than FP for three fold. Therefore CMC represented as an effective inducer for cellulase production. The feasibility of application of fermentable sugars to produce biodiesel were investigated in this study and described in details.
Patchanee Yasurin, Assumption University, Thailand
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