Biomass is the most abundant renewable resource in the world and has a potential use as an alternative to fossil resource for production of chemicals and fuels. One's of key challenges is the development of new processes for the utilization of biomass. For the effective conversion from biomass to biofuels or other chemicals, it requires efficient hydrolysis of cellulose to glucose or fermentable sugars at high yields. To improve the rate of hydrolysis of biomass by commercial cellulase enzyme, different pretreatment methods were performed and its efficiency was monitored and evaluated. Lignocellulosic biomass, rice straw, rice husk, and water hyacinth were pretreated with different chemicals, or pretreated with microwave heating, or with combination of chemicals and microwave heating. Pretreated biomass was saccharified by using commercial cellulase enzymes and sugar contents were measured. The combination of two pretreatment methods exhibited a synergy effect with 78.7% of the enzymatic sugar conversion. To study the possibility to utilize sugars from pretreated biomass, the de novo biosynthesis of fatty acid ethyl esters (FAEEs) by Acinetobacter spp were monitored. The key biochemical reaction is the esterification between fatty acyl Co-A and ethanol using diacylglycerol acyltransferase (DGAT). The preliminary experiments present here that bacterial growth phase has effect on FAEEs production and A.calcoaceticus is better option to use for further study as it can produce total FAEEs up to 1,070 mg/l from media containing sugars of pretreated biomass.
Malinee Sriariyanun, King's Mongkut University of Technology, Thailand
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