Biotyping of Jatropha Curcas L. from Thailand, Laos and Tanzania by MALDI-TOF MS Technique


It was shown that Jatropha curcas L. of the family Euphorbiaceae had significant economic importance for its seed oil, which could be used to substitute for diesel oil. It has been widely distributed in Thailand. This study was aimed at determining the biotype that could produce better oil content by using biotyping technique. It was found that among the biodiversity of 15 populations of J. curcas, Jc1 to Jc13 were obtained from different localities in Thailand, Jc 14 was obtained from Laos, and Jc15 was obtained from Tanzania. The biotyping technique, Matrix-Assisted Laser Desorption Ionization Time-of-Flight (MALDI-TOF) Mass Spectrometry method, was used for this purpose. The MALDI-TOF method was an inexpensive method that yielded rapidly with high accuracy results. Also, the results showed that the m/z spectrum pattern profiles of all populations were the same, and were confirmed with program ClinProTools 2.2. Therefore, it could be concluded that all populations (Jc1-15) were the same strain.

Author Information
Thitiporn Ditsawanon, Rajabhat Rajanagarindra University, Thailand
Sittiruk Roytrakul, Genome Institute, Thailand
Janthima Jaresittikulchai, Genome Institute, Thailand
Worawut Chulalaksananukul, Chulalongkorn University, Thailand

Paper Information
Conference: ACSEE2016
Stream: Energy: Renewable Energy and Environmental Solutions

This paper is part of the ACSEE2016 Conference Proceedings (View)
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Posted by James Alexander Gordon